中文摘要：

為了研究卵巢巨噬細胞群體在排卵中的作用，我們研究了使用脂質體封裝的氯膦酸鹽消耗該群體的影響。在排卵前 84 小時（第 -3 天）或 36 小時（第 -1 天）在促性腺激素引發(fā)的成年小鼠的卵巢滑囊下注射氯膦酸脂質體、鹽水脂質體或單獨生理鹽水。排卵率是通過計算釋放的卵母細胞數(shù)量來確定的。排卵前后也立即計算了graafian卵泡和黃體的數(shù)量。使用針對巨噬細胞抗原 macrosialin、主要組織相容性復合物 II 類 （Ia） 和 F4/80 的特異性單克隆抗體進行免疫組化檢查排卵前卵巢的 theca 和基質內的巨噬細胞分布。在第 -1 天注射氯膦酸脂質體不影響排卵率，而在第 -3 天注射氯膦酸脂質體導致排卵率顯著降低（平均排卵卵母細胞分別為 5. 25 +/- 0.6 來自氯膦酸脂質體處理的卵巢和 9.13 +/- 0.9 來自鹽水處理的卵巢，P < 0.05）。排卵時 theca 中存在的巨唾液酸陽性巨噬細胞的數(shù)量通過第 -1 天的氯膦酸脂質體處理減少，第 -3 天的治療減少了 theca 中存在的 Ia 陽性和巨唾液素陽性巨噬細胞的數(shù)量。當通過陰道涂片檢查隨后的卵巢周期時，發(fā)現(xiàn)氯膦酸脂質體處理的動物的中胚層-2/二元期延長（7.5 +/- 1.3天，鹽水脂質體處理動物為3.4 +/- 0.4天，P < 0.05）。這些結果表明，鞘狀巨噬細胞可能參與卵泡生長和破裂的調節(jié)，并且對發(fā)情周期的正常進展很重要。

英文摘要：

To investigate the role of the ovarian macrophage population in ovulation, we examined the effect of depleting this population using liposome-encapsulated clodronate. Clodronate liposomes, saline liposomes, or saline alone was injected under the ovarian bursa in gonadotropin-primed adult mice, either 84 h (Day -3) or 36 h (Day -1) before ovulation. Ovulation rates were determined by counting the number of oocytes released. The numbers of graafian follicles and corpora lutea were also counted immediately before and after ovulation. Macrophage distribution within the theca and stroma of preovulatory ovaries was examined by immunohistochemistry with specific monoclonal antibodies to the macrophage antigens macrosialin, major histocompatability complex class II (Ia), and F4/80. Injection of clodronate liposomes on Day -1 did not affect ovulation rates, whereas administration on Day -3 caused a significant reduction in ovulation rate (mean oocytes ovulated = 5. 25 +/- 0.6 from clodronate liposome-treated ovaries and 9.13 +/- 0.9 from saline-treated ovaries, respectively, P < 0.05). The numbers of macrosialin-positive macrophages present in the theca at ovulation were reduced by treatment with clodronate liposomes on Day -1, and treatment on Day -3 reduced the numbers of Ia-positive and macrosialin-positive macrophages present in the theca. When the subsequent ovarian cycles were examined by vaginal smearing, the metestrous-2/diestrous stage was found to be extended in clodronate liposome-treated animals (7.5 +/- 1.3 days vs. 3.4 +/- 0.4 days for saline liposome-treated animals, P < 0.05). These results suggest that thecal macrophages may be involved in the regulation of follicular growth and rupture, as well as being important for the normal progression of the estrous cycle.

產品詳情：

品牌：LIPOSOMA

貨號：CP-005-005
產地：荷蘭

品名：Clodronateliposomes氯膦酸二鈉脂質體巨噬細胞清除劑

實驗細節(jié)：

實驗結果：

論文題目：

ntrabursal injection of clodronate liposomes causes macrophage depletion and inhibits ovulation in the mouse ovary。

該文章比較久了，是較早研究卵巢巨噬細胞的文章，可以借鑒。因為研究很早，所以數(shù)據(jù)很基礎。無論如何，我們可以訂購荷蘭liposoma品牌的巨噬細胞清除劑Clodronateliposomes，具體訂購聯(lián)系靶點科技。這為研究清除卵巢巨噬細胞提供的研究基礎。